custom built tirf microscope Search Results


fluoview 500 confocal microscope  (Evident Corporation)
90
Evident Corporation fluoview 500 confocal microscope
Fluoview 500 Confocal Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluoview 500 confocal microscope/product/Evident Corporation
Average 90 stars, based on 1 article reviews
fluoview 500 confocal microscope - by Bioz Stars, 2026-04
90/100 stars
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custom-made tirf microscope visitirf  (VISITRON Inc)
90
VISITRON Inc custom-made tirf microscope visitirf
a , Binding of the β 1 -integrin-activation-reporting 9EG7 antibody in the presence of magnesium (white bars) or manganese (patterned bars) normalized to total β 1 -integrin levels. Dots represent experiments on different days (qKO, n = 5; dKO, n = 3; one-sample or two-sample t -test). b , <t>Total</t> <t>internal</t> <t>reflection</t> fluorescence <t>(TIRF)</t> microscopy images used for focal adhesion analysis of the indicated, serum-starved cells spread for 40 min on FN. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar, 10 µm. c , Average focal adhesion count and area per cell ( n = 4 independent experiments; >8 cells per condition; one-way ANOVA of repeated measurements with Tukey’s post hoc test) quantified from the mCherry–KIND2 signal. d , e , qKO-TLN1 WT KIND2 WT (black) and qKO-TLN1 K402E KIND2 WT (blue) cells ( d ) and dKO-TLN1 WT (gray) and dKO-TLN1 K402E (red) cells ( e ) attached to a concanavalin A-coated cantilever brought in contact with FNIII7–10 for the indicated contact times and separated with a loading rate of 5 µm s −1 . Dots represent separation forces of single fibroblasts, and lines indicate the median value. P values were calculated using two-tailed Mann–Whitney test and represent comparisons between qKO-TLN1 WT KIND2 WT and qKO-TLN1 K402E KIND2 WT cells or dKO-TLN1 WT and dKO-TLN1 K402E cells at the indicated contact times. f , g , Quantification of cell area from the TLN1–YPet signal 16 h after seeding serum-starved cells on FN, VN or laminin-111 ( f ) (laminin-111 (LN111), n = 3 independent experiments, >50 cells per condition) or FN-coated hydrogels with 4-, 12- or 50-kPa rigidity ( g ) ( n = 3 independent experiments, >50 cells per condition analyzed, one-way ANOVA of repeated measurements with Tukey’s post hoc test). For details on statistical methods and data representation, see .
Custom Made Tirf Microscope Visitirf, supplied by VISITRON Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom-made tirf microscope visitirf/product/VISITRON Inc
Average 90 stars, based on 1 article reviews
custom-made tirf microscope visitirf - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


a , Binding of the β 1 -integrin-activation-reporting 9EG7 antibody in the presence of magnesium (white bars) or manganese (patterned bars) normalized to total β 1 -integrin levels. Dots represent experiments on different days (qKO, n = 5; dKO, n = 3; one-sample or two-sample t -test). b , Total internal reflection fluorescence (TIRF) microscopy images used for focal adhesion analysis of the indicated, serum-starved cells spread for 40 min on FN. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar, 10 µm. c , Average focal adhesion count and area per cell ( n = 4 independent experiments; >8 cells per condition; one-way ANOVA of repeated measurements with Tukey’s post hoc test) quantified from the mCherry–KIND2 signal. d , e , qKO-TLN1 WT KIND2 WT (black) and qKO-TLN1 K402E KIND2 WT (blue) cells ( d ) and dKO-TLN1 WT (gray) and dKO-TLN1 K402E (red) cells ( e ) attached to a concanavalin A-coated cantilever brought in contact with FNIII7–10 for the indicated contact times and separated with a loading rate of 5 µm s −1 . Dots represent separation forces of single fibroblasts, and lines indicate the median value. P values were calculated using two-tailed Mann–Whitney test and represent comparisons between qKO-TLN1 WT KIND2 WT and qKO-TLN1 K402E KIND2 WT cells or dKO-TLN1 WT and dKO-TLN1 K402E cells at the indicated contact times. f , g , Quantification of cell area from the TLN1–YPet signal 16 h after seeding serum-starved cells on FN, VN or laminin-111 ( f ) (laminin-111 (LN111), n = 3 independent experiments, >50 cells per condition) or FN-coated hydrogels with 4-, 12- or 50-kPa rigidity ( g ) ( n = 3 independent experiments, >50 cells per condition analyzed, one-way ANOVA of repeated measurements with Tukey’s post hoc test). For details on statistical methods and data representation, see .

Journal: Nature Structural & Molecular Biology

Article Title: Talin and kindlin use integrin tail allostery and direct binding to activate integrins

doi: 10.1038/s41594-023-01139-9

Figure Lengend Snippet: a , Binding of the β 1 -integrin-activation-reporting 9EG7 antibody in the presence of magnesium (white bars) or manganese (patterned bars) normalized to total β 1 -integrin levels. Dots represent experiments on different days (qKO, n = 5; dKO, n = 3; one-sample or two-sample t -test). b , Total internal reflection fluorescence (TIRF) microscopy images used for focal adhesion analysis of the indicated, serum-starved cells spread for 40 min on FN. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar, 10 µm. c , Average focal adhesion count and area per cell ( n = 4 independent experiments; >8 cells per condition; one-way ANOVA of repeated measurements with Tukey’s post hoc test) quantified from the mCherry–KIND2 signal. d , e , qKO-TLN1 WT KIND2 WT (black) and qKO-TLN1 K402E KIND2 WT (blue) cells ( d ) and dKO-TLN1 WT (gray) and dKO-TLN1 K402E (red) cells ( e ) attached to a concanavalin A-coated cantilever brought in contact with FNIII7–10 for the indicated contact times and separated with a loading rate of 5 µm s −1 . Dots represent separation forces of single fibroblasts, and lines indicate the median value. P values were calculated using two-tailed Mann–Whitney test and represent comparisons between qKO-TLN1 WT KIND2 WT and qKO-TLN1 K402E KIND2 WT cells or dKO-TLN1 WT and dKO-TLN1 K402E cells at the indicated contact times. f , g , Quantification of cell area from the TLN1–YPet signal 16 h after seeding serum-starved cells on FN, VN or laminin-111 ( f ) (laminin-111 (LN111), n = 3 independent experiments, >50 cells per condition) or FN-coated hydrogels with 4-, 12- or 50-kPa rigidity ( g ) ( n = 3 independent experiments, >50 cells per condition analyzed, one-way ANOVA of repeated measurements with Tukey’s post hoc test). For details on statistical methods and data representation, see .

Article Snippet: The cells were imaged on a custom-made TIRF microscope (VisiTIRF, Visitron Systems) based on an Observer Z1 microscopy stand (Carl Zeiss).

Techniques: Binding Assay, Activation Assay, Fluorescence, Microscopy, Staining, Two Tailed Test, MANN-WHITNEY